Rescuers from the East Sussex Wildlife Rescue and Ambulance Service (WRAS) were first called out at 6.30am to a baby badger which had injured its foot in Hastings.
The animal was taken to WRAS's Casualty Care Centre in Horsebridge where she was examined by staff from Horsebridge vets.
Later in the morning, two calls came in about injured gulls in Eastbourne.
Rescuer Tony Neads attended an incident in Chiswick Place where a gull had been shot through the wing, while the charity's founder Trevor Weeks went to the aid of another which appeared to have been hit by a car outside Bishop Bell School.
Both gulls were taken to the Horsebridge centre where they were given X-rays.
Trevor was then called out to a fox cub found in a dustbin at the bottom of a garden in Eastbourne.
Fellow rescuer Barry Osbourne had managed to track down the cub after hearing it crying for two nights.
The cub was stuck in the bottom of an old-style round plastic dustbin hidden in bushes at the bottom of the garden.
Trevor said, "I'm not sure how the cub managed to get in there. There are some wood chippings piled up at one side but not high enough for the cub to get in. Mum would not have been able to reach in and get her cub. We felt we had no choice but to bring the little chap in and check him over, make sure he was OK and then try and get him back to his mum later in the night."
The cub was taken back to Horsebridge where he was checked over by veterinary staff before being bedded down for the rest of the day.
At 7.30pm the cub was delivered back to Eastbourne in the hope of being able to release it and reunite it with its mother.
Trevor said, "Unfortunately the wind was very strong and it was raining too so the conditions for the release were not looking good.
"By 3am we decided to call it a day – we were all very tired and were having difficulty keeping our eyes open.
"By 4.30am I was shattered and could hardly stay awake."
The fox cub was returned to Horsebridge and bedded down.
The entire day was filmed by a TV crew from BBC1's Animal 24:7 and these rescues should be aired later in the year.
Trevor said the exceptionally busy day was far from unusual.
He added, "We are almost 400 calls up on this time last year having dealt with more than 800 incidents so far this year.
"Our volunteers have been very dedicated and are invaluable. I certainly would not have been able to cope with this workload on my own like I used to. Without their help I would have burnt myself out by now.
"We have also had volunteers cleaning and feeding the casualties and our casualty care manager Tim has been busy all day looking after and feeding some of the more specialist animals in care too."
Trevor said dealing with Tuesday's casualties cost at least £400 in veterinary fees and motor expenses and the total volunteer time exceeded 57 hours.
Donations to the charity can be sent to WRAS's treasurer at Ash Cottage, 73 Friday Street, Eastbourne, East Sussex, BN23 8AY.
Video footage of some of the rescues can be viewed on the charity's website, www.wildlifeambulance.org or via YouTube at http://uk.youtube.com/user
/EastSussexWRAS